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Update RNAseq tutorial #5

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kescobo opened this issue Jun 8, 2022 · 4 comments · May be fixed by #10
Open

Update RNAseq tutorial #5

kescobo opened this issue Jun 8, 2022 · 4 comments · May be fixed by #10

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@kescobo
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kescobo commented Jun 8, 2022

Some work in this direction already in #2, though that's out of date now too. Also related to #3 .

@maltesie
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maltesie commented Apr 16, 2023

I made a draft for a new tutorial for RNAseq data. It uses reads from a TEX-treated sample in V. Cholerae and computes putative transcription start sites in it. It shows how to

  • download reads from SRA
  • align reads using bwa-mem
  • read .bam files and .gff files
  • annotate alignments with features from .gff files
  • compute coverage from alignments
  • compute maxima in the difference along the coverage as putative TSS

Does that make sense? I put everything here

@kescobo
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kescobo commented Apr 17, 2023

Go ahead an make a PR and we can discuss there. When you push to your fork, there should be a button that says "open pull request," but if not, you can open the pull requests tab here or on your repo, click "new PR", and then have your branch target main here:

image

@kescobo
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kescobo commented Apr 18, 2023

(if you'd like, I can kick off the PR from your fork, but it's good practice :-) )

@maltesie
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I created a PR, I think. Thanks for all the help :)

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2 participants