diff --git a/CHANGES.md b/CHANGES.md
index 12ef6cad..6491d90e 100644
--- a/CHANGES.md
+++ b/CHANGES.md
@@ -36,6 +36,13 @@ This release drops support for Python versions 3.6 and 3.7 and adds support for
   when the default shell is sh.
   ([#321](https://github.com/nextstrain/cli/pull/321))
 
+* The Singularity runtime once again supports Apptainer's `singularity`
+  executable.  The minimum version checking added in 7.0.0 broke usage of the
+  Singularity runtime with Apptainer (compared with SingularityCE).  Our intent
+  is to support both lineages of Singularity.  Thanks to @osageorange for
+  raising this issue and testing the fix!
+  ([#343](https://github.com/nextstrain/cli/pull/343))
+
 
 # 7.4.0 (21 September 2023)
 
diff --git a/nextstrain/cli/runner/singularity.py b/nextstrain/cli/runner/singularity.py
index 535fa361..6a238702 100644
--- a/nextstrain/cli/runner/singularity.py
+++ b/nextstrain/cli/runner/singularity.py
@@ -119,6 +119,8 @@
 
 SINGULARITY_MINIMUM_VERSION = "3.0.0"
 
+APPTAINER_MINIMUM_VERSION = "1.0.0" # forked from Singularity 3.9.5
+
 SINGULARITY_CONFIG_ENV = {
     # Store image caches in our runtime root instead of ~/.singularity/…
     "SINGULARITY_CACHEDIR": str(CACHE),
@@ -158,7 +160,7 @@ def SINGULARITY_EXEC_ARGS(): return [
     #     --writable-tmpfs        (3.0.0)
     #     --no-init               (3.0.0)
     #     --no-umask              (3.7.0)
-    #     --no-eval               (3.10.0)
+    #     --no-eval               (3.10.0, Apptainer 1.1.0)
     #
     # We opt not to use the --compat bundle option itself mainly for broader
     # version compatibility but also because what it includes will likely
@@ -208,7 +210,7 @@ def SINGULARITY_EXEC_ARGS(): return [
     # Don't evaluate the entrypoint command line (e.g. arguments passed via
     # `nextstrain build`) before exec-ing the entrypoint.  It leads to unwanted
     # substitutions that happen too early.
-    *(["--no-eval"] if singularity_version_at_least("3.10.0") else []),
+    *(["--no-eval"] if singularity_version_at_least("3.10.0", apptainer="1.1.0") else []),
 
     # Since we use --no-home above, avoid warnings about not being able to cd
     # to $HOME (the default behaviour).  run() will override this by specifying
@@ -335,8 +337,8 @@ def test_run():
     return [
         ("singularity is installed",
             shutil.which("singularity") is not None),
-        (f"singularity version {singularity_version()} ≥ {SINGULARITY_MINIMUM_VERSION}",
-            singularity_version_at_least(SINGULARITY_MINIMUM_VERSION)),
+        (f"singularity version {singularity_version()} ≥ {SINGULARITY_MINIMUM_VERSION} ({APPTAINER_MINIMUM_VERSION} for Apptainer)",
+            singularity_version_at_least(SINGULARITY_MINIMUM_VERSION, apptainer=APPTAINER_MINIMUM_VERSION)),
         ("singularity works",
             test_run()),
     ]
@@ -546,12 +548,15 @@ def run_bash(script: str, image: str = DEFAULT_IMAGE) -> List[str]:
 
 
 @lru_cache(maxsize = None)
-def singularity_version_at_least(min_version: str) -> bool:
+def singularity_version_at_least(min_version: str, *, apptainer: str) -> bool:
     version = singularity_version()
 
     if not version:
         return False
 
+    if singularity_is_apptainer():
+        min_version = apptainer
+
     return version >= Version(min_version)
 
 
@@ -571,3 +576,19 @@ def singularity_version() -> Optional[Version]:
             return Version(re.sub(r'-.+$', '', raw_version))
         except InvalidVersion:
             return None
+
+
+@lru_cache(maxsize = None)
+def singularity_is_apptainer() -> Optional[bool]:
+    singularity = shutil.which("singularity")
+
+    if not singularity:
+        return None
+
+    if not Path(singularity).is_symlink():
+        return False
+
+    try:
+        return Path(os.readlink(singularity)).name == "apptainer"
+    except OSError:
+        return None