diff --git a/src/bika/lims/browser/client/views/attachments.py b/src/bika/lims/browser/client/views/attachments.py
index 1f6a90cd34..12b5a1d2c5 100644
--- a/src/bika/lims/browser/client/views/attachments.py
+++ b/src/bika/lims/browser/client/views/attachments.py
@@ -77,7 +77,6 @@ def folderitem(self, obj, item, index):
         obj = api.get_object(obj)
         obj_url = api.get_url(obj)
         file = obj.getAttachmentFile()
-        icon = file.icon
         item['AttachmentFile'] = file.filename()
         item['AttachmentType'] = obj.getAttachmentType().Title()
         item['AttachmentType'] = obj.getAttachmentType().Title()
diff --git a/src/bika/lims/browser/reports/administration_arsnotinvoiced.py b/src/bika/lims/browser/reports/administration_arsnotinvoiced.py
index ab121e91d6..1d1a034486 100644
--- a/src/bika/lims/browser/reports/administration_arsnotinvoiced.py
+++ b/src/bika/lims/browser/reports/administration_arsnotinvoiced.py
@@ -41,7 +41,6 @@ def __init__(self, context, request, report=None):
     def __call__(self):
         bc = getToolByName(self.context, 'senaite_catalog')
         self.report_content = {}
-        parm_lines = {}
         parms = []
         headings = {}
         headings['header'] = _("Samples not invoiced")
@@ -86,11 +85,6 @@ def __call__(self):
         }
 
         datalines = []
-        clients = {}
-        sampletypes = {}
-        samplepoints = {}
-        categories = {}
-        services = {}
 
         for ar_proxy in bc(query):
             ar = ar_proxy.getObject()
diff --git a/src/bika/lims/browser/reports/productivity_analysespersampletype.py b/src/bika/lims/browser/reports/productivity_analysespersampletype.py
index b455dbe70c..e24ae45a63 100644
--- a/src/bika/lims/browser/reports/productivity_analysespersampletype.py
+++ b/src/bika/lims/browser/reports/productivity_analysespersampletype.py
@@ -44,7 +44,6 @@ def __call__(self):
         bac = getToolByName(self.context, 'senaite_catalog_analysis')
         rc = getToolByName(self.context, 'reference_catalog')
         self.report_content = {}
-        parm_lines = {}
         parms = []
         headings = {}
         headings['header'] = _("Analyses per sample type")
diff --git a/src/bika/lims/browser/widgets/scheduleinputwidget.py b/src/bika/lims/browser/widgets/scheduleinputwidget.py
index 5c09a590e6..873795e9c7 100644
--- a/src/bika/lims/browser/widgets/scheduleinputwidget.py
+++ b/src/bika/lims/browser/widgets/scheduleinputwidget.py
@@ -37,9 +37,7 @@ class ScheduleInputWidget(TypesWidget):
     security = ClassSecurityInfo()
     
     def process_form(self, instance, field, form, empty_marker=None, emptyReturnsMarker=False):
-        
-        values = len(instance.getScheduleCriteria())>0 and instance.getScheduleCriteria() or []
-        
+
         if "form.button.save" in form:
             value = []            
             fn = form['fieldName']
diff --git a/src/bika/lims/browser/worksheet/views/export.py b/src/bika/lims/browser/worksheet/views/export.py
index 1279003b3c..59968435c6 100644
--- a/src/bika/lims/browser/worksheet/views/export.py
+++ b/src/bika/lims/browser/worksheet/views/export.py
@@ -27,9 +27,6 @@ class ExportView(BrowserView):
     """
     """
     def __call__(self):
-
-        translate = self.context.translate
-
         instrument = self.context.getInstrument()
         if not instrument:
             self.context.plone_utils.addPortalMessage(
@@ -58,5 +55,5 @@ def __call__(self):
             return
 
         exporter = exim.Export(self.context, self.request)
-        data = exporter(self.context.getAnalyses())
+        exporter(self.context.getAnalyses())
         pass
diff --git a/src/bika/lims/content/instrument.py b/src/bika/lims/content/instrument.py
index 324a4a5c04..b734cb8adb 100644
--- a/src/bika/lims/content/instrument.py
+++ b/src/bika/lims/content/instrument.py
@@ -611,11 +611,7 @@ def addReferences(self, reference, service_uids):
         """
         # TODO Workflow - Analyses. Assignment of refanalysis to Instrument
         addedanalyses = []
-        wf = getToolByName(self, 'portal_workflow')
         bsc = getToolByName(self, 'senaite_catalog_setup')
-        bac = getToolByName(self, 'senaite_catalog_analysis')
-        ref_type = reference.getBlank() and 'b' or 'c'
-        ref_uid = reference.UID()
         postfix = 1
         for refa in reference.getReferenceAnalyses():
             grid = refa.getReferenceAnalysesGroupID()
diff --git a/src/bika/lims/content/instrumentmaintenancetask.py b/src/bika/lims/content/instrumentmaintenancetask.py
index 53de9b4395..49fdbea1d4 100644
--- a/src/bika/lims/content/instrumentmaintenancetask.py
+++ b/src/bika/lims/content/instrumentmaintenancetask.py
@@ -22,7 +22,6 @@
 from DateTime import DateTime
 from Products.Archetypes import atapi
 from Products.Archetypes.public import *
-from Products.CMFCore.utils import getToolByName
 from Products.CMFPlone.utils import safe_unicode
 from bika.lims import api
 from bika.lims import bikaMessageFactory as _
@@ -170,7 +169,6 @@ def getCurrentStateI18n(self):
         return safe_unicode(_(self.getCurrentState()).encode('utf-8'))
 
     def getCurrentState(self):
-        workflow = getToolByName(self, 'portal_workflow')
         if self.getClosed():
             return InstrumentMaintenanceTaskStatuses.CLOSED
         elif not api.is_active(self):
diff --git a/src/bika/lims/vocabularies/__init__.py b/src/bika/lims/vocabularies/__init__.py
index c4bc2fe625..e47424fda7 100644
--- a/src/bika/lims/vocabularies/__init__.py
+++ b/src/bika/lims/vocabularies/__init__.py
@@ -18,7 +18,6 @@
 # Copyright 2018-2021 by it's authors.
 # Some rights reserved, see README and LICENSE.
 
-from Acquisition import aq_get
 from bika.lims import bikaMessageFactory as _
 from bika.lims.api import is_active
 from bika.lims.utils import t
@@ -59,7 +58,6 @@ def __init__(self, context, key=None, value=None, contentFilter=None):
 
     def __call__(self, **kwargs):
         site = getSite()
-        request = aq_get(site, 'REQUEST', None)
         catalog = getToolByName(site, self.catalog)
         allow_blank = False
         if 'allow_blank' in kwargs:
@@ -106,9 +104,7 @@ def __init__(self, folders, portal_types):
 
     def __call__(self, context):
         site = getSite()
-        request = aq_get(site, 'REQUEST', None)
         items = []
-        wf = site.portal_workflow
         for folder in self.folders:
             folder = site.restrictedTraverse(folder)
             for portal_type in self.portal_types:
@@ -321,7 +317,6 @@ class ClientContactVocabulary(object):
 
     def __call__(self, context):
         site = getSite()
-        request = aq_get(site, 'REQUEST', None)
         items = []
         for client in site.clients.objectValues('Client'):
             objects = list(client.objectValues('Contact'))
diff --git a/src/senaite/core/browser/dashboard/dashboard.py b/src/senaite/core/browser/dashboard/dashboard.py
index 32b75bd1a7..9f124afbd1 100644
--- a/src/senaite/core/browser/dashboard/dashboard.py
+++ b/src/senaite/core/browser/dashboard/dashboard.py
@@ -663,7 +663,6 @@ def _getDateStr(self, period, created):
         elif period == PERIODICITY_MONTHLY:
             created = '%s-%s' % (str(created.year())[2:], str(created.month()).zfill(2))
         elif period == PERIODICITY_WEEKLY:
-            d = (((created.day()-1)/7)*7)+1
             year, weeknum, dow = created.asdatetime().isocalendar()
             created = created - dow
             created = '%s-%s-%s' % (str(created.year())[2:], str(created.month()).zfill(2), str(created.day()).zfill(2))
diff --git a/src/senaite/core/exportimport/instruments/facscalibur/calibur/model_e9750.py b/src/senaite/core/exportimport/instruments/facscalibur/calibur/model_e9750.py
index 4c07cc4c13..f48e556a88 100644
--- a/src/senaite/core/exportimport/instruments/facscalibur/calibur/model_e9750.py
+++ b/src/senaite/core/exportimport/instruments/facscalibur/calibur/model_e9750.py
@@ -37,8 +37,6 @@ def Import( context, request):
     fileformat = request.form['facs_calibur_format']
     artoapply = request.form['facs_calibur_artoapply']
     override = request.form['facs_calibur_override']
-    sample = request.form.get('facs_calibur_sample',
-                              'requestid')
     instrument = request.form.get('instrument', None)
     errors = []
     logs = []
@@ -70,16 +68,6 @@ def Import( context, request):
         elif override == 'overrideempty':
             over = [True, True]
 
-        sam = ['getId', 'getSampleID', 'getClientSampleID']
-        if sample == 'requestid':
-            sam = ['getId']
-        if sample == 'sampleid':
-            sam = ['getSampleID']
-        elif sample == 'clientsid':
-            sam = ['getClientSampleID']
-        elif sample == 'sample_clientsid':
-            sam = ['getSampleID', 'getClientSampleID']
-
         importer = FacsCalibur2Importer(parser=parser,
                                         context=context,
                                         allowed_ar_states=status,
diff --git a/src/senaite/core/exportimport/instruments/foss/fiastar/fiastar.py b/src/senaite/core/exportimport/instruments/foss/fiastar/fiastar.py
index ee1479b4a4..9c86b1cef6 100644
--- a/src/senaite/core/exportimport/instruments/foss/fiastar/fiastar.py
+++ b/src/senaite/core/exportimport/instruments/foss/fiastar/fiastar.py
@@ -49,7 +49,6 @@ class Export(BrowserView):
     def __call__(self, analyses):
         tray = 1
         now = DateTime().strftime('%Y%m%d-%H%M')
-        bsc = getToolByName(self.context, 'senaite_catalog_setup')
         uc = getToolByName(self.context, 'uid_catalog')
         instrument = self.context.getInstrument()
         norm = getUtility(IIDNormalizer).normalize
diff --git a/src/senaite/core/exportimport/instruments/foss/winescan/ft120.py b/src/senaite/core/exportimport/instruments/foss/winescan/ft120.py
index 9fb5f418a6..d98c79091b 100644
--- a/src/senaite/core/exportimport/instruments/foss/winescan/ft120.py
+++ b/src/senaite/core/exportimport/instruments/foss/winescan/ft120.py
@@ -158,7 +158,6 @@ def _addRawResult(self, resid, values={}, override=False):
         # First, we must find if already exists a row with results for
         # the same date, in order to take into account replicas, Mean
         # and Standard Deviation
-        dtidx = values.get('Calibration',{}).get('Calibration',0)
         rows = self.getRawResults().get(resid, [])
         row, rows = self._extractrowbycalibration(rows, self._calibration)
         is_std = values.get('Rep #',{}).get('Rep #','') == 'Sd'
diff --git a/src/senaite/core/exportimport/instruments/instrument.py b/src/senaite/core/exportimport/instruments/instrument.py
index 277aeef3b2..adc01f3aae 100644
--- a/src/senaite/core/exportimport/instruments/instrument.py
+++ b/src/senaite/core/exportimport/instruments/instrument.py
@@ -34,7 +34,6 @@ def getResultsInputFile(request):
 
 def GenericImport(context, request, parser, importer=None):
     infile = getResultsInputFile(request)
-    fileformat = getFileFormat(request)
     artoapply = request.form['artoapply']
     override = request.form['results_override']
 
diff --git a/src/senaite/core/exportimport/instruments/panalytical/omnia/__init__.py b/src/senaite/core/exportimport/instruments/panalytical/omnia/__init__.py
index 2bf953d13a..e08b34d3bd 100644
--- a/src/senaite/core/exportimport/instruments/panalytical/omnia/__init__.py
+++ b/src/senaite/core/exportimport/instruments/panalytical/omnia/__init__.py
@@ -129,8 +129,6 @@ def parse_resultline(self, line):
         # Split by ","
         splitted = self.splitLine(line.strip(";"))
 
-        errors = ''
-
         # Adjunt separated values from split by ','
         for idx, result in enumerate(splitted):
             if result.startswith('"'):
diff --git a/src/senaite/core/exportimport/setupdata/__init__.py b/src/senaite/core/exportimport/setupdata/__init__.py
index 3cbb7930d2..b73cb1e301 100644
--- a/src/senaite/core/exportimport/setupdata/__init__.py
+++ b/src/senaite/core/exportimport/setupdata/__init__.py
@@ -515,7 +515,6 @@ def Import(self):
 class Lab_Products(WorksheetImporter):
 
     def Import(self):
-        context = self.context
         # Refer to the default folder
         folder = self.context.bika_setup.bika_labproducts
         # Iterate through the rows
@@ -609,7 +608,7 @@ def Import(self):
             password = safe_unicode(row['Password']).encode('utf-8')
             if(username):
                 try:
-                    member = self.context.portal_registration.addMember(
+                    self.context.portal_registration.addMember(
                         username,
                         password,
                         properties={
@@ -1223,8 +1222,6 @@ class Storage_Locations(WorksheetImporter):
 
     def Import(self):
         setup_folder = self.context.bika_setup.bika_storagelocations
-        bsc = getToolByName(self.context, 'senaite_catalog_setup')
-        pc = getToolByName(self.context, 'portal_catalog')
         for row in self.get_rows(3):
             if not row['Address']:
                 continue
@@ -1688,7 +1685,6 @@ def resolve_service(self, row):
         return service
 
     def Import(self):
-        s_t = ""
         bucket = {}
         pc = getToolByName(self.context, "portal_catalog")
         bsc = getToolByName(self.context, "senaite_catalog_setup")
@@ -2156,7 +2152,6 @@ def load_reference_analysis_interims(self, analysis):
             if not worksheet:
                 return
             self.interim_worksheet = worksheet
-        bsc = getToolByName(self.context, 'senaite_catalog_setup')
         interims = []
         for row in self.get_rows(3, worksheet=self.interim_worksheet):
             if row['ReferenceAnalysis_id'] != analysis.getId():
@@ -2260,8 +2255,6 @@ def load_analysis_interims(self, analysis):
         analysis.setInterimFields(interims)
 
     def Import(self):
-        bc = getToolByName(self.context, 'senaite_catalog')
-        bsc = getToolByName(self.context, 'senaite_catalog_setup')
         pc = getToolByName(self.context, 'portal_catalog')
         for row in self.get_rows(3):
             if not row['id']:
diff --git a/travis_ci_flake8.cfg b/travis_ci_flake8.cfg
index 0f7c521727..b4d7ea6a5c 100644
--- a/travis_ci_flake8.cfg
+++ b/travis_ci_flake8.cfg
@@ -79,8 +79,6 @@ extend-ignore =
     F812,
     # F821: undefined name 'Report'
     F821,
-    # F841: local variable 'icon' is assigned to but never used
-    F841,
     # F901: 'raise NotImplemented' should be 'raise NotImplementedError'
     F901,
     # W191: indentation contains tabs